| Day 0 - Sunday 04-09-2022 |
|
|
| TIME |
PROGRAM |
ACTIVITIES |
TUTOR |
| In
advance |
Pre-Course
Seminar |
"The very Basics" in nucleic acids amplification for
Molecular Diagnostics: principles, biochemistry and their applications |
Will be provided online 2
weeks in advance |
| 4-6 pm |
Question
Hour |
Answering all open
questions |
all Tutors |
| Labwork |
Peripheral blood
sampling |
| Day 1 - Monday 05-09-2022 |
|
|
| TIME |
PROGRAM |
ACTIVITIES |
TUTOR |
| 09:00
am - 10:00 am |
Introduction |
Introduction to course
issues
the IFCC-EMD and C-CMBC group primary and secondary objectives of
course
Introduction to Workshop, organisation and in-silico training |
V.
Haselmann |
| 10:00
am - 11:00 am |
Today's
program |
Lecture and video on DNA
isolation |
J.
Schmid-Burgk |
| 11:00
am - 11:30 am |
Labwork |
1. Introduction of today's workshop issues building students for
the groups
2. preparation of buffers and working solutions (1h) DNA isolation (part I,
1h). |
all Tutors |
| 11:30
am - 01:00 pm |
| 01:00
pm - 02:00 pm |
LUNCH BREAK |
| 02:00
pm - 02:45 pm |
Seminar |
Molecular
diagnostics of genetic disorders |
O. Díez Gibert |
| 03:00 pm - 04:00 pm |
Labwork |
3. DNA isolation (part II,
1-1,5h) |
all Tutors |
| 04:00
pm - 04:30 pm |
COFFEE BREAK |
| 04:30 pm - 06:00 pm |
Seminar |
Molecular
Genetic Testing (MGT); Guidelines for "Good Molecular Practice in
MGT"; Quality Assurance, EQA and Accreditation |
V. Haselmann |
| 6:00 pm - 06:30 pm |
Seminar |
Experience
of last course and benefits of being a junior member; Overview of MolDx in
Bolivia and what happend after the course |
R. Pessoa Rejas |
| Day 2 - Tuesday 06-09-2022 |
|
|
| TIME |
PROGRAM |
ACTIVITIES |
TUTOR |
| 09:00
am - 10:00 am |
Seminar |
DNA quantitation &
today's program |
A.
Ferreira-Gonzalez |
| 10:00
am - 12:00 pm |
Labwork |
1. prepare gel buffers
(15min)
2. prepare an 1% agarose gel (group1)
(30min)
3. check of gDNA content (UV-spectrophotometer) (30min)
4. set-up HPV-PCR (30min)
5. load the 1% agarose gel (30min, each student loads his own gDNA) |
all Tutors |
| 12:00 am - 01:00 pm |
LUNCH BREAK |
| 01:00
pm - 03:00 pm |
In silico |
in-silico
training on PCR assay design (optional DISCUSSIONS for HOMEWORK) |
V. Haselmann / all tutors |
| 03:00
pm - 03:30 pm |
COFFEE BREAK |
| 03:30
pm - 04:30 pm |
Seminar |
Nomeclature |
V. Haselmann |
| 04:30 pm - 05:30 pm |
Seminar |
Interpretation
of genetic variants |
A. Ferreira-Gonzalez |
| 05:45 pm - 06:45 pm |
Seminar |
Molecular
Oncology |
O. Díez Gibert |
| 06:45 pm - 07:00 pm |
Evaluation |
Discussion
of experimental results |
V.
Haselmann |
| Day 3 - Wednsday 07-09-2022 |
|
|
| TIME |
PROGRAM |
ACTIVITIES |
TUTOR |
| 09:00
am - 09:30 am |
Today's
program |
Introduction to PCR and
specifics of amplification protocols - PCR Movie |
J.
Schmid-Burgk |
| 09:30
am - 12:00 pm |
Labwork |
1. BRAF ASA (start 10:00,
finish 12:00)
2. prepare 2% agarose gel (group 2 - one big gel, two combs) for HPV-PCR
and 2% gel for BRAF (group 3 - one gel, two combs)
3. load agarose gel with HPV-PCR
4. discussion of homeworks
5. HLA B57 PCR set-up
// Trouble-shooting PCR |
all Tutors |
| 12:00 pm - 01:00 pm |
LUNCH BREAK |
| 01:00 pm - 02:00 pm |
Labwork |
6. load
BRAF PCR, run gel
7. set-up 2.5% agarose-gel for HLA
B57 (group 4 - one gel, two combs), gel will be load and run by tutors |
all Tutors |
| 02:00
pm - 03:30 pm |
In silico |
in-silico training: a)
discussion of homeworks b) setting up RFLP assay c) BLAST |
V. Haselmann / all tutors |
| 03:30 pm - 04:00 pm |
COFFEE BREAK |
| 04:00 pm - 05:15 pm |
Seminar |
SNP
genotyping |
V. Haselmann |
| 05:15
pm - 06:30 pm |
Seminar |
Concept of targeted
therapies - examples for breast and colon cancer (BRAC, bTMB) |
O. Díez
Gibert |
| 06:30
pm - 07:00 pm |
Evaluation |
discussion of
electrophoresis results |
V. Haselmann |
| Day 4 Thursday 08-09-2022 |
|
|
| TIME |
PROGRAM |
ACTIVITIES |
TUTOR |
| 09:00
am - 09:30 am |
Today's
program |
Discussion on RFLP in
silico design |
O. Díez
Gibert |
| 09:30 am - 11:30 am |
Labwork |
1. set-up an RFLP assay
Factor V and RFLP assay for LCT
2. prepare three 2% agarose gel for LCT/FV undigested reaction (group 5 -
one gel, two combs)
3. discussion of electropherograms |
all Tutors |
| 11:30
am - 12:30 pm |
LUNCH BREAK |
| 12:30 pm - 01:30 pm |
Labwork |
4. set-up
of restriction digestion
5. load and run gels for undigested reaction
6. prepare 2% agarose gel (one gel, two combs) for digested reaction, gel
will be load and run by tutors |
all Tutors |
| 01:30 pm - 03:00 pm |
Seminar |
Validation of assays |
A. Ferreira-Gonzalez |
| 03:00 pm - 04:00 pm |
Seminar |
Pharmacogenetics |
V.
Haselmann |
| 04:00
pm - 04:30 pm |
COFFEE BREAK |
| 04:30
pm - 05:30 pm |
Seminar |
New
detection methods in Molecular Diagnostics: LAMP |
J. Schmid-Burgk |
| 05:30 pm - 06:00 pm |
Evaluation |
Discussion of experimental
results |
V.
Haselmann |
| 06:00 pm - 07:00 pm |
|
Interviews with candidates
applying as junior member |
all Tutors |
| Day 5 - Friday 09-09-2022 |
|
|
| 09:00 am - 10:00 am |
Seminar |
Real time PCR
quantification aspects, LODs, LOQs and High Resolution Melting
analysis |
A. Ferreira-Gonzalez |
| 10:00 am - 11:30 am |
Labwork |
1) real time PCR for
genotyping , Factor V or something different |
all Tutors |
| |
|
|
|
| 11:30
am - 12:30 pm |
LUNCH BREAK |
| 12:30
pm - 13:00 pm |
In silico |
Probe
design |
O. Díez
Gibert / V. Haselmann |
| 13:00 pm - 14:00 pm |
In silico |
Determination of LOD and LOQ by students |
all tutors |
| 14:00 pm - 15:00 pm |
Seminar |
Infectious diseases |
A. Ferreira-Gonzalez |
| Day 6 - Saturday 10-09-2022 |
|
|
| TIME |
PROGRAM |
ACTIVITIES |
TUTOR |
| 09:00
am - 10:30 am |
Evaluation |
Exam (30
questions) & Evaluation of the course |
all
tutors |
| 10:30
am - 11:00 am |
COFFEE BREAK |
| 11:00 am - 12:00 am |
Seminar |
State-of-the-Art
Lecture: Liquid Biopsy |
V. Haselmann |
| 12:00
am -01:00 pm |
Seminar |
State-of-the-Art Lecture:
Next Generation Sequencing |
J.
Schmid-Burgk |
| 13:00
- 14:00 |
LUNCH BREAK |
| 02:00
pm - 03:00 pm |
Fare Well |
Results of Exams, Issuing
Certificates, Nomination of Junior Member |
V.
Haselmann |
|
|
|
|